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Phaya Nak or Phaya Nāga (Thai: พญานาค; RTGS: phaya nak; lit. 'lord of Nāga', phaya derived from Mon which indicate superior nobility) or Nakkharat (Thai: นาคราช; lit. 'king of Nāga') in Thai beliefs, nagas are viewed as the patrons of water. Nagas are thought to live in possibly water bodies or in caves. In accordance with a popular legend, the Mekong River in north-jap Thailand and Laos was said to get developed by two Nāga kings slithering through the region, Consequently creating the Mekong as well as close by Nan River.
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The loss of action is not as a result of a diminished ability to bind divalent cations For the reason that metallic content from the purified mutants was identical to the wild sort enzyme. The catalytic Attributes of these mutants are completely in line with the proposed purpose of the residue within the chemical mechanism as the principal common base for activation in the hydrolytic drinking water molecule and the following protonation with the leaving group amine.
A female naga is referred to as a "nagi" or "nagini". A nagaraja is a king from the nagas.[three] They can be popular in Indian mythology and Enjoy a very important purpose in several spiritual traditions.
Right now 1.five mL of acetic anhydride was included and allowed to respond at area temperature right away. naga 26 Right after removing in the solvent, the residue was dissolved in a hundred mL of chloroform and washed with dilute HCl, saturated NaHCO3 then dried about Na2SO4. The product, diphenyl N
Mainly because of the solid relation with all the things h2o, the Nāga in Thai perception also performs a task in rain Management. The notion of Nak hai nam (Thai: นาคให้น้ำ; lit. Nāga granting h2o) is employed for yearly rainfall prediction.
Additionally it is a base healthcare facility in the Helen Keller Foundation, where by eye professionals from all around the state are experienced and later on assigned to diverse portions of the Philippines.[sixty one]
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Reconstitution of the apo-enzymes was executed overnight at four °C in advance of elimination of unbound metal by passage via a PD-10 column. The PD-ten column was pre-addressed with dipicolinate to remove traces of unbound metal and then washed with five column volumes of metallic free of charge HEPES buffer, pH eight.0. After elution, the concentration on the metal-reconstituted enzyme was determined by UV absorbance plus the metallic content material with the samples determined by ICP-MS.